Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA.
Identifieur interne : 003A02 ( Main/Exploration ); précédent : 003A01; suivant : 003A03Genetic and alkaloid analysis of Papaver species and their F1 hybrid by RAPD, HPLC and ELISA.
Auteurs : Y. Shoyama [Japon] ; F. Kawachi ; H. Tanaka ; R. Nakai ; T. Shibata ; K. NishiSource :
- Forensic science international [ 0379-0738 ] ; 1998.
Descripteurs français
- KwdFr :
- ADN des plantes (analyse), Alcaloïdes (analyse), Amorces ADN (), Chromatographie en phase liquide à haute performance, Chromosomes (génétique), Marqueurs biologiques (analyse), Papaver (), Papaver (génétique), Plantes médicinales, Spécificité d'espèce, Stupéfiants (), Technique RAPD, Test ELISA, Thébaïne ().
- MESH :
English descriptors
- KwdEn :
- Alkaloids (analysis), Biomarkers (analysis), Chromatography, High Pressure Liquid, Chromosomes (genetics), DNA Primers (chemistry), DNA, Plant (analysis), Enzyme-Linked Immunosorbent Assay, Narcotics (chemistry), Papaver (chemistry), Papaver (genetics), Plants, Medicinal, Random Amplified Polymorphic DNA Technique, Species Specificity, Thebaine (chemistry).
- MESH :
- chemical , analysis : Alkaloids, Biomarkers, DNA, Plant.
- chemical , chemistry : DNA Primers, Narcotics, Thebaine.
- chemistry : Papaver.
- genetics : Chromosomes, Papaver.
- Chromatography, High Pressure Liquid, Enzyme-Linked Immunosorbent Assay, Plants, Medicinal, Random Amplified Polymorphic DNA Technique, Species Specificity.
Abstract
Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.
DOI: 10.1016/s0379-0738(97)00196-5
PubMed: 9530831
Affiliations:
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Shibata</name></author><author><name sortKey="Nishi, K" sort="Nishi, K" uniqKey="Nishi K" first="K" last="Nishi">K. Nishi</name></author></analytic><series><title level="j">Forensic science international</title><idno type="ISSN">0379-0738</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alkaloids (analysis)</term><term>Biomarkers (analysis)</term><term>Chromatography, High Pressure Liquid</term><term>Chromosomes (genetics)</term><term>DNA Primers (chemistry)</term><term>DNA, Plant (analysis)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Narcotics (chemistry)</term><term>Papaver (chemistry)</term><term>Papaver (genetics)</term><term>Plants, Medicinal</term><term>Random Amplified Polymorphic DNA Technique</term><term>Species Specificity</term><term>Thebaine (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN des plantes (analyse)</term><term>Alcaloïdes (analyse)</term><term>Amorces ADN ()</term><term>Chromatographie en phase liquide à haute performance</term><term>Chromosomes (génétique)</term><term>Marqueurs biologiques (analyse)</term><term>Papaver ()</term><term>Papaver (génétique)</term><term>Plantes médicinales</term><term>Spécificité d'espèce</term><term>Stupéfiants ()</term><term>Technique RAPD</term><term>Test ELISA</term><term>Thébaïne ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Alkaloids</term><term>Biomarkers</term><term>DNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA Primers</term><term>Narcotics</term><term>Thebaine</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN des plantes</term><term>Alcaloïdes</term><term>Marqueurs biologiques</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Papaver</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes</term><term>Papaver</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chromosomes</term><term>Papaver</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Plants, Medicinal</term><term>Random Amplified Polymorphic DNA Technique</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Chromatographie en phase liquide à haute performance</term><term>Papaver</term><term>Plantes médicinales</term><term>Spécificité d'espèce</term><term>Stupéfiants</term><term>Technique RAPD</term><term>Test ELISA</term><term>Thébaïne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Total DNA was extracted from the leaves of a F1 hybrid and its parents, Papaver bracteatum Lindle and P. pseudo-orientale Medw. Analysis of random-amplified polymorphic DNA (RAPD) using ten arbitrary oligonucleotide 10-mers, showed that F1 hybrid was confirmed to be genetically intermediate of both parental plants compared with the genetic distance between F1 hybrid and individual parents. Furthermore, the comparison of the band patterns between a F1 hybrid, P. bracteatum and P. pseudo-orientale clearly showed that part of the bands of both parents were induced into a F1 hybrid. The content of thebaine was determined by HPLC and ELISA used anti-thebaine monoclonal antibody.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Kyūshū</li><li>Préfecture de Fukuoka</li></region><settlement><li>Fukuoka</li></settlement><orgName><li>Université de Kyūshū</li></orgName></list><tree><noCountry><name sortKey="Kawachi, F" sort="Kawachi, F" uniqKey="Kawachi F" first="F" last="Kawachi">F. Kawachi</name><name sortKey="Nakai, R" sort="Nakai, R" uniqKey="Nakai R" first="R" last="Nakai">R. Nakai</name><name sortKey="Nishi, K" sort="Nishi, K" uniqKey="Nishi K" first="K" last="Nishi">K. Nishi</name><name sortKey="Shibata, T" sort="Shibata, T" uniqKey="Shibata T" first="T" last="Shibata">T. Shibata</name><name sortKey="Tanaka, H" sort="Tanaka, H" uniqKey="Tanaka H" first="H" last="Tanaka">H. Tanaka</name></noCountry><country name="Japon"><region name="Kyūshū"><name sortKey="Shoyama, Y" sort="Shoyama, Y" uniqKey="Shoyama Y" first="Y" last="Shoyama">Y. Shoyama</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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